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Control and monitoring of parameters in microfluidic systems
Meloun, Jan ; Sekora, Jiří (referee) ; Čmiel, Vratislav (advisor)
This thesis discusses the construction of Microfluidic systems for a microscope. The theoretical part of this thesis deals with light, light’s behaviour and optical spectral analysis in some methods are discussed. Also, the use of the development platform Arduino with wireless data transfer for data visualization is mentioned. Methods for measuring pH are mentioned as well. The practical part is dedicated to the construction of the Microfluidic system with the use of Arduino, smart mobile devices and a developed mobile application including controlling liquid flow with the measured data visualization.
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The cell migration monitoring in a microfluidic system by the "Scratch Wound Healing Assay" method
Morgaenko, Katsiarina ; Skopalík, Josef (referee) ; Chmelíková, Larisa (advisor)
Tato diplomová práce se zabývá popisem principů kultivace embryonálních fibroblastových buněk myší (3T3), lidských endoteliálních buněk odebraných z pupečníkové žily (HUVEC) a epiteliálních buněk vaječníku čínského křečka (CHO) v mikrofluidních systémech simulujících kapiláry. Byly provedeny literární rešerše v oblasti realizací experimentu “Scratch Wound Healing Assay” v mikrofluidních systémech s použitím fibroblastů a endotheliálních buněk. V práci jsou dále popsány principy konfokální a fluorescenční mikroskopie a metody zpracování obrazů pro sledování buněčné migrace. Experimentální nastavení pro mikrofluidní realizaci “Scratch Wound Healing Assay” s použitím trypsinu – EDTA pro vytvoření rýhy, a konfokálního mikroskopu Leica TCS SP8 X pro následující snímání pořízených dat bylo navrženo a otestováno s dostatečným počtem opakování. Vhodný algoritmus pro analýzu buněčné migrace byl napsán v programovacím prostředí Matlab. Závěrem této práce je diskuze získaných výsledků.
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Flow measurement in microfluidic systems with recording and sharing of measured values
Duša, Martin ; Harabiš, Vratislav (referee) ; Čmiel, Vratislav (advisor)
This paper is focused on flow measurement, microfluidic systems and realization system, which is able to flow measure and share with the possibility of their visualization via web interface. The first part of the work is devoted to the theoretical research, which briefly deals with the theory of flow and other concepts that belong to the flow There are also mentioned principles of flowmeter and their advantages or applications. The second part is devoted to microfluidic system. This section describes the elements that make up the microfluidic system, their capabilities and applications. The last part is devoted to the realization of the measuring system. The implementation includes a design of the measuring system, where the selection of microcotroler and sensor is given. The software solution includes used platforms and examples of algorithmization. At the end, the physical implementation of the whole system and calibration is described.
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Control and monitoring of parameters in microfluidic systems
Meloun, Jan ; Sekora, Jiří (referee) ; Čmiel, Vratislav (advisor)
This thesis discusses the construction of Microfluidic systems for a microscope. The theoretical part of this thesis deals with cell and its survival in the environment. Furthermore, light, light’s behavior and optical spectral analysis in some methods is discussed. Also, the use of the development platform Arduino with wireless data transfer for data visualization is mentioned. Methods for measuring pH are mentioned as well. The practical part is dedicated to the construction of the Microfluidic system with the use of Arduino, smart mobile devices and a developed mobile application including controlling liquid flow with the measured data visualization.
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|
Flow measurement in microfluidic systems with recording and sharing of measured values
Duša, Martin ; Harabiš, Vratislav (referee) ; Čmiel, Vratislav (advisor)
This paper is focused on flow measurement, microfluidic systems and realization system, which is able to flow measure and share with the possibility of their visualization via web interface. The first part of the work is devoted to the theoretical research, which briefly deals with the theory of flow and other concepts that belong to the flow There are also mentioned principles of flowmeter and their advantages or applications. The second part is devoted to microfluidic system. This section describes the elements that make up the microfluidic system, their capabilities and applications. The last part is devoted to the realization of the measuring system. The implementation includes a design of the measuring system, where the selection of microcotroler and sensor is given. The software solution includes used platforms and examples of algorithmization. At the end, the physical implementation of the whole system and calibration is described.
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|
|
The cell migration monitoring in a microfluidic system by the "Scratch Wound Healing Assay" method
Morgaenko, Katsiarina ; Skopalík, Josef (referee) ; Chmelíková, Larisa (advisor)
Tato diplomová práce se zabývá popisem principů kultivace embryonálních fibroblastových buněk myší (3T3), lidských endoteliálních buněk odebraných z pupečníkové žily (HUVEC) a epiteliálních buněk vaječníku čínského křečka (CHO) v mikrofluidních systémech simulujících kapiláry. Byly provedeny literární rešerše v oblasti realizací experimentu “Scratch Wound Healing Assay” v mikrofluidních systémech s použitím fibroblastů a endotheliálních buněk. V práci jsou dále popsány principy konfokální a fluorescenční mikroskopie a metody zpracování obrazů pro sledování buněčné migrace. Experimentální nastavení pro mikrofluidní realizaci “Scratch Wound Healing Assay” s použitím trypsinu – EDTA pro vytvoření rýhy, a konfokálního mikroskopu Leica TCS SP8 X pro následující snímání pořízených dat bylo navrženo a otestováno s dostatečným počtem opakování. Vhodný algoritmus pro analýzu buněčné migrace byl napsán v programovacím prostředí Matlab. Závěrem této práce je diskuze získaných výsledků.
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|
|
Control and monitoring of parameters in microfluidic systems
Meloun, Jan ; Sekora, Jiří (referee) ; Čmiel, Vratislav (advisor)
This thesis discusses the construction of Microfluidic systems for a microscope. The theoretical part of this thesis deals with light, light’s behaviour and optical spectral analysis in some methods are discussed. Also, the use of the development platform Arduino with wireless data transfer for data visualization is mentioned. Methods for measuring pH are mentioned as well. The practical part is dedicated to the construction of the Microfluidic system with the use of Arduino, smart mobile devices and a developed mobile application including controlling liquid flow with the measured data visualization.
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